HCSGD entry for EP300

1. General information

Official gene symbolEP300
Entrez ID2033
Gene full nameE1A binding protein p300
Other gene symbolsKAT3B RSTS2 p300
Links to Entrez GeneLinks to Entrez Gene

2. Neighbors in the network

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3. Gene ontology annotation

GO ID

GO term

Evidence

Category

GO:0000122Negative regulation of transcription from RNA polymerase II promoterIDAbiological_process
GO:0000123Histone acetyltransferase complexIEAcellular_component
GO:0000785ChromatinIEAcellular_component
GO:0000978RNA polymerase II core promoter proximal region sequence-specific DNA bindingIEAmolecular_function
GO:0001047Core promoter bindingIDAmolecular_function
GO:0001102RNA polymerase II activating transcription factor bindingIPImolecular_function
GO:0001666Response to hypoxiaIDAbiological_process
GO:0001756SomitogenesisIEAbiological_process
GO:0001889Liver developmentIEAbiological_process
GO:0001934Positive regulation of protein phosphorylationIEAbiological_process
GO:0002039P53 bindingIEAmolecular_function
GO:0003677DNA bindingIDAmolecular_function
GO:0003682Chromatin bindingIMPmolecular_function
GO:0003713Transcription coactivator activityIDAmolecular_function
GO:0003823Antigen bindingIEAmolecular_function
GO:0004402Histone acetyltransferase activityIDAmolecular_function
GO:0004468Lysine N-acetyltransferase activityIDAmolecular_function
GO:0005515Protein bindingIPImolecular_function
GO:0005634NucleusIDAcellular_component
GO:0005654NucleoplasmTAScellular_component
GO:0005667Transcription factor complexIEAcellular_component
GO:0005730NucleolusIDAcellular_component
GO:0005737CytoplasmIDAcellular_component
GO:0006351Transcription, DNA-templatedIEAbiological_process
GO:0006355Regulation of transcription, DNA-templatedIDAbiological_process
GO:0006915Apoptotic processIMPbiological_process
GO:0007049Cell cycleIEAbiological_process
GO:0007219Notch signaling pathwayTASbiological_process
GO:0007399Nervous system developmentTASbiological_process
GO:0007507Heart developmentIEAbiological_process
GO:0007519Skeletal muscle tissue developmentIEAbiological_process
GO:0008013Beta-catenin bindingIPImolecular_function
GO:0008134Transcription factor bindingIPImolecular_function
GO:0008270Zinc ion bindingIEAmolecular_function
GO:0009749Response to glucoseIEAbiological_process
GO:0009887Organ morphogenesisIEAbiological_process
GO:0010560Positive regulation of glycoprotein biosynthetic processIEAbiological_process
GO:0010942Positive regulation of cell deathIEAbiological_process
GO:0016032Viral processIEAbiological_process
GO:0016407Acetyltransferase activityIMPmolecular_function
GO:0016746Transferase activity, transferring acyl groupsIDAmolecular_function
GO:0018076N-terminal peptidyl-lysine acetylationIDAbiological_process
GO:0018393Internal peptidyl-lysine acetylationIDAbiological_process
GO:0030324Lung developmentIEAbiological_process
GO:0031490Chromatin DNA bindingIEAmolecular_function
GO:0032025Response to cobalt ionIEAbiological_process
GO:0032092Positive regulation of protein bindingIEAbiological_process
GO:0032403Protein complex bindingIEAmolecular_function
GO:0032481Positive regulation of type I interferon productionTASbiological_process
GO:0032526Response to retinoic acidIEAbiological_process
GO:0032967Positive regulation of collagen biosynthetic processIEAbiological_process
GO:0032993Protein-DNA complexIEAcellular_component
GO:0033160Positive regulation of protein import into nucleus, translocationIEAbiological_process
GO:0033613Activating transcription factor bindingIPImolecular_function
GO:0034612Response to tumor necrosis factorIEAbiological_process
GO:0035259Glucocorticoid receptor bindingIEAmolecular_function
GO:0042493Response to drugIEAbiological_process
GO:0042771Intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediatorIDAbiological_process
GO:0042975Peroxisome proliferator activated receptor bindingIEAmolecular_function
GO:0043388Positive regulation of DNA bindingIEAbiological_process
GO:0043425BHLH transcription factor bindingIEAmolecular_function
GO:0043491Protein kinase B signalingIEAbiological_process
GO:0043627Response to estrogenIDAbiological_process
GO:0043923Positive regulation by host of viral transcriptionIDAbiological_process
GO:0043967Histone H4 acetylationIMPbiological_process
GO:0043969Histone H2B acetylationIDAbiological_process
GO:0045087Innate immune responseTASbiological_process
GO:0045471Response to ethanolIEAbiological_process
GO:0045727Positive regulation of translationIEAbiological_process
GO:0045773Positive regulation of axon extensionIEAbiological_process
GO:0045793Positive regulation of cell sizeIEAbiological_process
GO:0045862Positive regulation of proteolysisIEAbiological_process
GO:0045944Positive regulation of transcription from RNA polymerase II promoterIDA IMPbiological_process
GO:0046332SMAD bindingIEAmolecular_function
GO:0048565Digestive tract developmentIEAbiological_process
GO:0050681Androgen receptor bindingIPImolecular_function
GO:0050714Positive regulation of protein secretionIEAbiological_process
GO:0051019Mitogen-activated protein kinase bindingIEAmolecular_function
GO:0051059NF-kappaB bindingIEAmolecular_function
GO:0051091Positive regulation of sequence-specific DNA binding transcription factor activityIDAbiological_process
GO:0051384Response to glucocorticoidIEAbiological_process
GO:0051592Response to calcium ionIEAbiological_process
GO:0060177Regulation of angiotensin metabolic processIEAbiological_process
GO:0060298Positive regulation of sarcomere organizationIEAbiological_process
GO:0060765Regulation of androgen receptor signaling pathwayIDAbiological_process
GO:0061418Regulation of transcription from RNA polymerase II promoter in response to hypoxiaTASbiological_process
GO:0065004Protein-DNA complex assemblyIEAbiological_process
GO:0070301Cellular response to hydrogen peroxideIEAbiological_process
GO:0070542Response to fatty acidIEAbiological_process
GO:0071407Cellular response to organic cyclic compoundIEAbiological_process
GO:0071456Cellular response to hypoxiaTASbiological_process
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4. Expression levels in datasets

  • Meta-analysis result

p-value upp-value downFDR upFDR down
0.00445782830.98469421370.17751059001.0000000000

  • Individual experiment result
    ( "-" represent NA in the specific microarray platform )

Data sourceUp or downLog fold change
GSE11954Down-0.0436148452
GSE13712_SHEARUp0.4367711935
GSE13712_STATICDown-0.0004451624
GSE19018Up0.0849735290
GSE19899_A1Up0.6520193462
GSE19899_A2Up0.5278268257
PubMed_21979375_A1Up0.4059391148
PubMed_21979375_A2Up1.8586821277
GSE35957Up0.0113602914
GSE36640Up0.0683780452
GSE54402Up0.2502883993
GSE9593Up0.0117940370
GSE43922Up0.3038224698
GSE24585Up0.3557914004
GSE37065Up0.2030013788
GSE28863_A1Up1.4979453868
GSE28863_A2Up1.2436181457
GSE28863_A3Up0.1950723400
GSE28863_A4Down-0.0244027795
GSE48662Down-0.2917802436

5. Regulation relationships with compounds/drugs/microRNAs

  • Compounds

Not regulated by compounds

  • Drugs

Not regulated by drugs

  • MicroRNAs

    • mirTarBase

MiRNA_name

mirBase ID

miRTarBase ID

Experiment

Support type

References (Pubmed ID)

hsa-miR-200c-3pMIMAT0000617MIRT003598MicroarrayFunctional MTI (Weak)19569050
hsa-miR-200b-3pMIMAT0000318MIRT003599MicroarrayFunctional MTI (Weak)19569050
hsa-miR-429MIMAT0001536MIRT003600MicroarrayFunctional MTI (Weak)19569050
hsa-miR-182-5pMIMAT0000259MIRT003601MicroarrayFunctional MTI (Weak)19569050
hsa-miR-194-5pMIMAT0000460MIRT003603MicroarrayFunctional MTI (Weak)19569050
hsa-miR-26b-5pMIMAT0000083MIRT003604MicroarrayFunctional MTI (Weak)19569050
hsa-miR-374a-5pMIMAT0000727MIRT004566MicroarrayFunctional MTI (Weak)19569050
hsa-miR-150-5pMIMAT0000451MIRT007087Luciferase reporter assayFunctional MTI23211718
hsa-miR-9-5pMIMAT0000441MIRT021346MicroarrayFunctional MTI (Weak)17612493
hsa-miR-130b-5pMIMAT0004680MIRT038305CLASHFunctional MTI (Weak)23622248
hsa-miR-615-3pMIMAT0003283MIRT039708CLASHFunctional MTI (Weak)23622248
hsa-miR-181d-5pMIMAT0002821MIRT041181CLASHFunctional MTI (Weak)23622248
hsa-miR-193b-3pMIMAT0002819MIRT041490CLASHFunctional MTI (Weak)23622248
hsa-miR-149-5pMIMAT0000450MIRT045469CLASHFunctional MTI (Weak)23622248
hsa-miR-140-5pMIMAT0000431MIRT045823CLASHFunctional MTI (Weak)23622248
hsa-miR-23b-3pMIMAT0000418MIRT046334CLASHFunctional MTI (Weak)23622248
hsa-miR-181b-5pMIMAT0000257MIRT047254CLASHFunctional MTI (Weak)23622248
hsa-miR-30c-5pMIMAT0000244MIRT047948CLASHFunctional MTI (Weak)23622248
hsa-miR-92a-3pMIMAT0000092MIRT049743CLASHFunctional MTI (Weak)23622248
hsa-miR-30a-3pMIMAT0000088MIRT049923CLASHFunctional MTI (Weak)23622248
hsa-miR-25-3pMIMAT0000081MIRT050220CLASHFunctional MTI (Weak)23622248
hsa-let-7b-5pMIMAT0000063MIRT051995CLASHFunctional MTI (Weak)23622248
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    • mirRecord
No target information from mirRecord

6. Text-mining results about the gene

Gene occurances in abstracts of cellular senescence-associated articles: 17 abstracts the gene occurs.


PubMed ID of the article

Sentenece the gene occurs

26629991It was shown that HN incubation triggered persistent downregulation of deacetylase SIRT1 and upregulation of acetyltransferase p300, leading to sustained hyperacetylation (at K382) and activation of p53, and subsequent p53/p21-mediated senescent "memory
26629991Interestingly, we found that SIRT1 and p300 could regulate each other in response to HN stimulation, suggesting that a delicate balance between acetyltransferases and deacetylases may be particularly important for sustained acetylation and activation of non-histone proteins (such as p53), and eventually the occurrence of "metabolic memory
25815136Epigenetic regulators such as p300, protein inhibitors of activated Stats and H19 help maintain stem cell quiescence
24488586The transcription factors predicted to regulate these changes, Nf-kappaB, Myb, Nkx2-1, Nr5a2, and Ep300, are known to regulate inflammation, differentiation, lipid metabolism, and chromatin remodeling, all of which have previously been implicated in aging
24458358These observations were paralleled by a downregulation of the GCN5-related N-acetyltransferases (GNAT) p300/CBP-associated factor and its isoform 5-alpha general control of amino acid synthesis (GCN5a), determining a relative decrease in total HAT activity
24218884Comparative Q-PCR was utilized to investigate target gene (P66SHC, EP300, HDAC1) expressions respectively in H2O2 treated groups and normal cell groups
24218884CONCLUSION: P66SHC, EP300 and HDAC1 probably play a role in cellular replicative senescence and oxidative-stress inducing premature senescence
23698071Selective inhibition of p300 HAT blocks cell cycle progression, induces cellular senescence, and inhibits the DNA damage response in melanoma cells
23698071Global assessment of the p300 HAT transcriptome in human melanoma identified functional roles in promoting cell cycle progression, chromatin assembly, and activation of DNA repair pathways through direct transcriptional regulatory mechanisms
23698071Together, our data suggest that p300 HAT activity mediates critical growth regulatory pathways in tumor cells and may serve as a potential therapeutic target for melanoma and other malignancies by promoting cellular responses to DNA damaging agents that are currently ineffective against specific cancers
23342163High glucose induced alteration of SIRTs in endothelial cells causes rapid aging in a p300 and FOXO regulated pathway
23342163Relationship of such alteration with histone acetylase (HAT) p300 was examined
23342163We also found that p300 and SIRT1 regulate each other in such process, as silencing one led to increase of the others' expression
23342163Chemically induced increased SIRT1 activity and p300 knockdown corrected these abnormalities slowing aging-like changes
23342163Data from this study demonstrated that hyperglycemia accelerates aging-like process in the vascular ECs and such process is mediated via downregulation of SIRT1, causing reduction of mitochondrial antioxidant enzyme in a p300 and FOXO1 mediated pathway
22731250The p300 CH3 domain is essential for both the autoacetylation of p300 and transference of the acetyl group to p53 and HDAC1 is a component of several non-p300 complexes that enhance p53 deacetylation, ubiquitination, and proteosomal degradation
22731250HDAC1 can also bind the p300-CH3 domain, regulating p300 acetylation and interfering with p300 mediated p53 acetylation
22731250The importance of this pathway is illustrated by showing complete restoration of p53 acetylation and partial restoration of p300 acetylation by treating SPRR2A expressing cells with HDAC1 siRNA
22095030In addition, ING proteins are thought to interact with and modulate the function of auxiliary members of p53 pathway, such as MDM2, ARF , p300, and p21, indicating their widespread involvement in the regulation and function of this prominent tumor suppressor pathway
21868306METHODS: In primary human fibroblasts, the proteins regulating cell growth in association with E1A NH(2) terminus, including the Rb family proteins, p300/CBP, and p400, were inactivated or interfered
21868306RESULTS: The Inactivation of Rb family proteins alone was not sufficient to rescue ras-induced cell senescence, whereas inactivation of both the Rb proteins and p300/CBP blocked ras-induced senescence of human fibroblasts
21868306CONCLUSION: Rb and p300/CBP binding activities are both required for E1A to bypass ras-induced senescence in human fibroblasts
20660480We propose that PPARgamma is subject to regulation by acetylation and deacetylation via p300 and SIRT1 in cellular senescence
17898049Treatment of E7-expressing cells with interferon ultimately resulted in cellular senescence through a process that is dependent upon acetylation of p53 by p300/CBP at lysine 382
17898049Cells expressing mutant forms of E6 that are unable to bind p300/CBP or bind p53 failed to block acetylation of p53 at lysine 382 and were sensitive to growth arrest by interferon
17898049This study identifies an important physiological role for E6 binding to p300/CBP in blocking growth arrest of human keratinocytes in the presence of interferon and so contributes to the persistence of HPV-infected cells
17452980Expression of the N-terminal domain of hAda3 that binds p53 but not p300 blocked p14ARF-induced p53 acetylation and protected MECs from senescence
16516887Relocalization occurs concomitantly with interaction with a subset of nuclear proteins, including PCNA, p53 and several regulators of acetylation such as the p300/CBP and PCAF histone acetyltransferases (HATs), as well as the histone deacetylases HDAC1 and hSir2
15892717Importantly, a promoter-reporter analysis demonstrated that the coactivator p300/CBP switched MITF from a repressor to an activator of DCT transcription
9467855Also, we have previously found that an increase in the level of the down stream target of p53 protein, p21WAF1/SD11/C1P1, is dependent on both p53 and p300 proteins
9312059We show that the DNA binding and transcriptional activity of p53 protein increases with cell age in the absence of any marked increase in the level of p53 protein, and that p21(WAF1) promoter activity in senescent cells is dependent on both p53 and the transcriptional co-activator p300
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