HCSGD entry for DHFR
1. General information
| Official gene symbol | DHFR |
|---|---|
| Entrez ID | 1719 |
| Gene full name | dihydrofolate reductase |
| Other gene symbols | DHFRP1 DYR |
| Links to Entrez Gene | Links to Entrez Gene |
2. Neighbors in the network
3. Gene ontology annotation
GO ID | GO term | Evidence | Category |
|---|---|---|---|
| GO:0000082 | G1/S transition of mitotic cell cycle | TAS | biological_process |
| GO:0000083 | Regulation of transcription involved in G1/S transition of mitotic cell cycle | TAS | biological_process |
| GO:0000278 | Mitotic cell cycle | TAS | biological_process |
| GO:0003729 | MRNA binding | IDA | molecular_function |
| GO:0004146 | Dihydrofolate reductase activity | IDA IEA NAS | molecular_function |
| GO:0005575 | Cellular_component | ND | cellular_component |
| GO:0005654 | Nucleoplasm | TAS | cellular_component |
| GO:0005829 | Cytosol | TAS | cellular_component |
| GO:0006545 | Glycine biosynthetic process | IEA | biological_process |
| GO:0006730 | One-carbon metabolic process | IEA | biological_process |
| GO:0006766 | Vitamin metabolic process | TAS | biological_process |
| GO:0006767 | Water-soluble vitamin metabolic process | TAS | biological_process |
| GO:0008144 | Drug binding | IDA | molecular_function |
| GO:0009165 | Nucleotide biosynthetic process | IEA | biological_process |
| GO:0031427 | Response to methotrexate | IEA | biological_process |
| GO:0044281 | Small molecule metabolic process | TAS | biological_process |
| GO:0046209 | Nitric oxide metabolic process | TAS | biological_process |
| GO:0046653 | Tetrahydrofolate metabolic process | IDA | biological_process |
| GO:0046654 | Tetrahydrofolate biosynthetic process | IEA | biological_process |
| GO:0046655 | Folic acid metabolic process | TAS | biological_process |
| GO:0050661 | NADP binding | IEA | molecular_function |
| GO:0050999 | Regulation of nitric-oxide synthase activity | TAS | biological_process |
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4. Expression levels in datasets
- Meta-analysis result
| p-value up | p-value down | FDR up | FDR down |
|---|---|---|---|
| 0.9957944759 | 0.0001417081 | 0.9999902473 | 0.0209544944 |
- Individual experiment result
( "-" represent NA in the specific microarray platform )
( "-" represent NA in the specific microarray platform )
| Data source | Up or down | Log fold change |
|---|---|---|
| GSE11954 | Down | -1.0879266808 |
| GSE13712_SHEAR | Down | -0.2022905733 |
| GSE13712_STATIC | Down | -0.4647943987 |
| GSE19018 | Down | -0.3856528268 |
| GSE19899_A1 | Down | -1.3368908128 |
| GSE19899_A2 | Down | -1.8579305401 |
| PubMed_21979375_A1 | Down | -2.1188518733 |
| PubMed_21979375_A2 | Down | -2.6649036720 |
| GSE35957 | Down | -1.8279164686 |
| GSE36640 | Down | -3.0289244840 |
| GSE54402 | Down | -0.5165933217 |
| GSE9593 | Down | -1.2855856776 |
| GSE43922 | Down | -0.8899915339 |
| GSE24585 | Up | 0.1643743761 |
| GSE37065 | Down | -0.2728251337 |
| GSE28863_A1 | Down | -0.0940942457 |
| GSE28863_A2 | Up | 0.0299274750 |
| GSE28863_A3 | Down | -0.0962345618 |
| GSE28863_A4 | Down | -0.1640178888 |
| GSE48662 | Down | -0.4781075389 |
5. Regulation relationships with compounds/drugs/microRNAs
- Compounds
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- Drugs
Name | Drug | Accession number |
|---|---|---|
| NADH | DB00157 | NUTR00041 | DB01907 | EXPT02287 | DB03527 |
| Trimethoprim | DB00440 | APRD00103 |
| Pemetrexed | DB00642 | APRD00573 | EXPT02075 |
| Gentamicin | DB00798 | APRD00214 |
| Proguanil | DB01131 | APRD00188 |
| Trimetrexate | DB01157 | APRD00268 |
| 2,4-Diamino-5-Methyl-6-[(3,4,5-Trimethoxy-N-Methylanilino)Methyl]Pyrido[2,3-D]Pyrimidine | DB02104 | EXPT00976 |
| 2,4-Diamino-6-[N-(2',5'-Dimethoxybenzyl)-N-Methylamino]Quinazoline | DB02427 | EXPT00983 |
| 6-(Octahydro-1h-Indol-1-Ylmethyl)Decahydroquinazoline-2,4-Diamine | DB02559 | EXPT03103 |
| N6-(2,5-Dimethoxy-Benzyl)-N6-Methyl-Pyrido[2,3-D]Pyrimidine-2,4,6-Triamine | DB02583 | EXPT02658 |
| 2,4-Diamino-6-[N-(3',4',5'-Trimethoxybenzyl)-N-Methylamino]Pyrido[2,3-D]Pyrimidine | DB02919 | EXPT01286 |
| Sri-9662 | DB03060 | EXPT02032 |
| 2,4-Diamino-5-(3,4,5-Trimethoxy-Benzyl)-Pyrimidin-1-Ium | DB03125 | EXPT03114 |
| Sri-9439 | DB03351 | EXPT02031 |
| 2'-Monophosphoadenosine 5'-Diphosphoribose | DB03461 | EXPT02293 |
| 6-(2,5-Dimethoxy-Benzyl)-5-Methyl-Pyrido[2,3-D]Pyrimidine-2,4-Diamine | DB03695 | EXPT02259 |
| Biopterin | DB03886 | EXPT00708 |
| 2,4-Diamino-6-[N-(3',5'-Dimethoxybenzyl)-N-Methylamino]Pyrido[2,3-D]Pyrimidine | DB03987 | EXPT00988 |
| 5-[(3R)-3-(5-methoxybiphenyl-3-yl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine | DB07140 | - |
| 5-[(3R)-3-(5-methoxy-4'-methylbiphenyl-3-yl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine | DB07141 | - |
| 5-[(3R)-3-(5-methoxy-3',5'-dimethylbiphenyl-3-yl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine | DB07142 | - |
| 5-[(3R)-3-(5-methoxy-2',6'-dimethylbiphenyl-3-yl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine | DB07144 | - |
| 5-[3-(2,5-dimethoxyphenyl)prop-1-yn-1-yl]-6-ethylpyrimidine-2,4-diamine | DB08234 | - |
| [N-(2,4-DIAMINOPTERIDIN-6-YL)-METHYL]-DIBENZ[B,F]AZEPINE | DB08406 | - |
| (4aS)-5-[(2,4-diaminopteridin-6-yl)methyl]-4a,5-dihydro-2H-dibenzo[b,f]azepin-8-ol | DB08448 | - |
| (2R,6S)-6-{[methyl(3,4,5-trimethoxyphenyl)amino]methyl}-1,2,5,6,7,8-hexahydroquinazoline-2,4-diamine | DB08642 | - |
- MicroRNAs
- mirTarBase
- mirTarBase
MiRNA_name | mirBase ID | miRTarBase ID | Experiment | Support type | References (Pubmed ID) |
|---|---|---|---|---|---|
| hsa-miR-24-3p | MIMAT0000080 | MIRT002018 | Western blot//Luciferase reporter assay | Functional MTI | 17686970 |
| hsa-miR-24-3p | MIMAT0000080 | MIRT002018 | Microarray | Functional MTI (Weak) | 19748357 |
| hsa-miR-935 | MIMAT0004978 | MIRT036640 | CLASH | Functional MTI (Weak) | 23622248 |
| hsa-miR-93-3p | MIMAT0004509 | MIRT038796 | CLASH | Functional MTI (Weak) | 23622248 |
| hsa-miR-503-5p | MIMAT0002874 | MIRT041132 | CLASH | Functional MTI (Weak) | 23622248 |
| hsa-miR-99b-5p | MIMAT0000689 | MIRT044182 | CLASH | Functional MTI (Weak) | 23622248 |
| hsa-miR-15b-5p | MIMAT0000417 | MIRT046435 | CLASH | Functional MTI (Weak) | 23622248 |
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- mirRecord
- mirRecord
MicroRNA name | mirBase ID | Target site number | MiRNA mature ID | Test method inter | MiRNA regulation site | Reporter target site | Pubmed ID |
|---|---|---|---|---|---|---|---|
| hsa-miR-24-3p | MIMAT0000080 | 1 | hsa-miR-24 | {Western blot} | {overexpression by miRNA mimics transfection} | 17686970 | |
| hsa-miR-192-5p | MIMAT0000222 | NA | hsa-miR-192 | {Western blot} | {overexpression by miRNA precursor transfection} | 19088023 | |
| hsa-miR-24-3p | MIMAT0000080 | NA | hsa-miR-24 | {Western blot} | {overexpression by miRNA precursor transfection} | 20041160 |
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6. Text-mining results about the gene
Gene occurances in abstracts of cellular senescence-associated articles: 7 abstracts the gene occurs.
PubMed ID of the article | Sentenece the gene occurs |
|---|---|
| 10799836 | This difference is much higher than those observed for classical enzymatic targets of chemotherapy such as thymidylate synthetase, dihydrofolate reductase and topoisomerases |
| 10739678 | Gene-specific repair was analyzed during cellular aging in the dihydrofolate reductase housekeeping gene, the p53 tumor suppressor gene, and the inactive region X(754) |
| 10585259 | The regulation of thymidine kinase (TK) and dihydrofolate reductase (DHFR) genes in human diploid fibroblasts serves as an example of how NF-Y may have a role in replicative senescence by regulating age-dependent G1/S genes |
| 9281303 | Since we have shown that thymidine kinase (TK) and dihydrofolate reductase (DHFR) are transcriptionally regulated during aging, we suspect that a similar mechanism may be operative in the age-dependent down-regulation of other G1/S genes |
| 9281303 | DNA binding activities using Y-box containing sequence in TK promoter or E2F containing sequence in DHFR promoter show prominent serum-responsiveness in low passage cells and dramatic attenuation in senescent cells |
| 9281303 | Specifically, these genes can be divided into two groups, one group contains tandem multiple CCAAT element, similar to that in TK promoter and the other contains E2F site, similar to that in DHFR promoter |
| 8816912 | Regulation of dihydrofolate reductase gene expression and E2F components in human diploid fibroblasts during growth and senescence |
| 8816912 | The induction of dihydrofolate reductase (DHFR), a key enzyme in DNA biosynthesis that is induced just before the onset of S phase, is markedly attenuated in senescent human fibroblasts (Pang and Chen, 1994, J |
| 8816912 | Gel mobility shift assays were performed to assess the role of each cis-element in the regulation of DHFR gene expression |
| 8077291 | The enzymes encoded by TS and DHFR genes and dUTPase also exhibited similar age-dependent attenuation in activities |
| 8206919 | Senescent cells were also markedly deficient in E2F binding activity associated with the dihydrofolate reductase promoter |
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