HCSGD entry for POLA1
1. General information
| Official gene symbol | POLA1 |
|---|---|
| Entrez ID | 5422 |
| Gene full name | polymerase (DNA directed), alpha 1, catalytic subunit |
| Other gene symbols | POLA p180 |
| Links to Entrez Gene | Links to Entrez Gene |
2. Neighbors in the network
This gene isn't in Literature mining network.
3. Gene ontology annotation
GO ID | GO term | Evidence | Category |
|---|---|---|---|
| GO:0000082 | G1/S transition of mitotic cell cycle | TAS | biological_process |
| GO:0000083 | Regulation of transcription involved in G1/S transition of mitotic cell cycle | TAS | biological_process |
| GO:0000084 | Mitotic S phase | IDA | biological_process |
| GO:0000166 | Nucleotide binding | IDA IEA | molecular_function |
| GO:0000278 | Mitotic cell cycle | TAS | biological_process |
| GO:0000722 | Telomere maintenance via recombination | TAS | biological_process |
| GO:0000723 | Telomere maintenance | TAS | biological_process |
| GO:0000731 | DNA synthesis involved in DNA repair | IMP | biological_process |
| GO:0000785 | Chromatin | IDA | cellular_component |
| GO:0001882 | Nucleoside binding | IEA | molecular_function |
| GO:0003677 | DNA binding | IDA IEA NAS | molecular_function |
| GO:0003682 | Chromatin binding | IDA | molecular_function |
| GO:0003887 | DNA-directed DNA polymerase activity | IDA IEA IMP | molecular_function |
| GO:0003896 | DNA primase activity | IDA | molecular_function |
| GO:0005515 | Protein binding | IPI | molecular_function |
| GO:0005634 | Nucleus | IDA | cellular_component |
| GO:0005635 | Nuclear envelope | IDA | cellular_component |
| GO:0005654 | Nucleoplasm | IDA TAS | cellular_component |
| GO:0005658 | Alpha DNA polymerase:primase complex | IDA TAS | cellular_component |
| GO:0005730 | Nucleolus | IDA | cellular_component |
| GO:0005737 | Cytoplasm | IDA | cellular_component |
| GO:0006260 | DNA replication | IMP | biological_process |
| GO:0006269 | DNA replication, synthesis of RNA primer | IDA | biological_process |
| GO:0006270 | DNA replication initiation | IDA TAS | biological_process |
| GO:0006271 | DNA strand elongation involved in DNA replication | IMP TAS | biological_process |
| GO:0006272 | Leading strand elongation | IDA | biological_process |
| GO:0006273 | Lagging strand elongation | IDA | biological_process |
| GO:0006281 | DNA repair | IDA | biological_process |
| GO:0006297 | Nucleotide-excision repair, DNA gap filling | IBA | biological_process |
| GO:0006303 | Double-strand break repair via nonhomologous end joining | IMP | biological_process |
| GO:0008283 | Cell proliferation | IDA | biological_process |
| GO:0008408 | 3'-5' exonuclease activity | IBA | molecular_function |
| GO:0016032 | Viral process | IEA | biological_process |
| GO:0016363 | Nuclear matrix | IDA | cellular_component |
| GO:0019985 | Translesion synthesis | IBA | biological_process |
| GO:0032201 | Telomere maintenance via semi-conservative replication | TAS | biological_process |
| GO:0032774 | RNA biosynthetic process | IDA | biological_process |
| GO:0043231 | Intracellular membrane-bounded organelle | IDA | cellular_component |
| GO:0046872 | Metal ion binding | IEA | molecular_function |
| GO:0046982 | Protein heterodimerization activity | IEA | molecular_function |
| GO:0051539 | 4 iron, 4 sulfur cluster binding | IEA | molecular_function |
| GO:0090305 | Nucleic acid phosphodiester bond hydrolysis | IBA | biological_process |
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4. Expression levels in datasets
- Meta-analysis result
| p-value up | p-value down | FDR up | FDR down |
|---|---|---|---|
| 0.9722436572 | 0.0003420083 | 0.9999902473 | 0.0339560150 |
- Individual experiment result
( "-" represent NA in the specific microarray platform )
( "-" represent NA in the specific microarray platform )
| Data source | Up or down | Log fold change |
|---|---|---|
| GSE11954 | Down | -0.1652143693 |
| GSE13712_SHEAR | Down | -0.4112997712 |
| GSE13712_STATIC | Down | -0.0972529026 |
| GSE19018 | Down | -0.2916023914 |
| GSE19899_A1 | Down | -0.3324440637 |
| GSE19899_A2 | Down | -4.5099394909 |
| PubMed_21979375_A1 | Down | -2.6870697685 |
| PubMed_21979375_A2 | Down | -3.1501682591 |
| GSE35957 | Down | -1.2185066148 |
| GSE36640 | Down | -3.6218671574 |
| GSE54402 | Down | -0.5268182520 |
| GSE9593 | Down | -1.1465865900 |
| GSE43922 | Down | -1.2287975277 |
| GSE24585 | Down | -0.3340057142 |
| GSE37065 | Down | -0.2334754046 |
| GSE28863_A1 | Up | 0.3867545170 |
| GSE28863_A2 | Up | 0.8035178865 |
| GSE28863_A3 | Down | -0.3220159374 |
| GSE28863_A4 | Up | 0.1937569853 |
| GSE48662 | Down | -0.6733257519 |
5. Regulation relationships with compounds/drugs/microRNAs
- Compounds
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- MicroRNAs
- mirTarBase
MiRNA_name | mirBase ID | miRTarBase ID | Experiment | Support type | References (Pubmed ID) |
|---|---|---|---|---|---|
| hsa-miR-193b-3p | MIMAT0002819 | MIRT016282 | Microarray | Functional MTI (Weak) | 20304954 |
| hsa-miR-124-3p | MIMAT0000422 | MIRT022295 | Microarray | Functional MTI (Weak) | 18668037 |
| hsa-miR-26b-5p | MIMAT0000083 | MIRT028969 | Microarray | Functional MTI (Weak) | 19088304 |
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- mirRecord
No target information from mirRecord
6. Text-mining results about the gene
Gene occurances in abstracts of cellular senescence-associated articles: 12 abstracts the gene occurs.
PubMed ID of the article | Sentenece the gene occurs |
|---|---|
| 12067597 | Replicative enzymes and ageing: importance of DNA polymerase alpha function to the events of cellular ageing |
| 12067597 | In eukaryotic cells, ranging from yeast to man, a replicative enzyme essential for initiation of transcription is DNA polymerase alpha (pol alpha), the activity of which is coordinately regulated with the initiation of DNA synthesis |
| 11707897 | Activity of DNA polymerase alpha in aging human fibroblasts |
| 10885806 | Aging and DNA polymerase alpha: modulation by dietary restriction |
| 10515663 | DNA polymerase alpha and the 3'-->5' exonuclease involved in the proofreading of DNA synthesis were isolated from human diploid fetal lung fibroblast (TIG-1) cells at various population doubling levels (PDL) |
| 10515663 | The fidelity of DNA polymerase alpha remained high until late passage and fell suddenly just before the end of the life span between 65 and 69 PDL |
| 10515663 | In vitro DNA synthesis by DNA polymerase alpha from TIG-1 cells harvested at 69 PDL showed the amount of non-complementary nucleotides incorporated to be decreased by the addition of the 3'-->5' exonuclease from the same cells |
| 10515663 | However, not all errors were edited out since the ratio of DNA polymerase activity to 3'-->5' exonuclease activity was adjusted to reflect that in vivo and the infidelity of DNA synthesis by error-prone DNA polymerase alpha from aged cells was improved by the addition of the highly active 3'-->5' exonuclease from cells at 41 PDL |
| 10227381 | The activity of these extracts was compared after supplementation with three recombinant human replication factors: (1) the catalytic subunit of DNA polymerase alpha (DNA pol-alpha-cat), (2) the three subunits of replication protein A (RPA), and (3) DNA topoisomerase I (Topo I) |
| 10227381 | Taken together, these results indicate that the low to undetectable activity of young HF extracts in this system is due mainly to reduced intracellular levels of RPA, while the senescent HF extracts are relatively deficient in DNA polymerase alpha and probably some other essential replication factors, as well as RPA |
| 8106564 | Murine temperature-sensitive DNA polymerase alpha mutant displays a diminished capacity to stimulate DNA synthesis in senescent human fibroblast nuclei in heterokaryons at the nonpermissive condition |
| 8106564 | We have investigated the capacity of a murine cell line with a temperature-sensitive (ts) mutation in the DNA polymerase alpha (Pola) locus and a series of ts non-Pola mutant cell lines from separate complementation groups to stimulate DNA synthesis, in senescent fibroblast nuclei in heterokaryons |
| 1988288 | DNA polymerase alpha and the regulation of entry into S phase in heterokaryons |
| 1988288 | In the studies described here, we have used cell fusion technology to investigate the formal kinetic relationship between the concentration of DNA polymerase alpha and the rate of reinitiation of DNA synthesis in nuclei from senescent cells |
| 1988288 | Heterokaryons were derived from the fusion of senescent cells to a series of actively dividing cell types with inherently different DNA polymerase alpha activities per cell |
| 1988288 | A kinetic analysis revealed a first-order relationship between the entry into S phase of senescent nuclei and the concentration of DNA polymerase alpha activity calculated to be in heterokaryons |
| 2632279 | The relationship between cell size, the activity of DNA polymerase alpha and proliferative activity in human diploid fibroblast-like cell cultures |
| 2632279 | In kinetic studies with human diploid fibroblast-like (HDFL) cells carried out in heterokaryons and in monokaryons, we have observed a first-order relationship between the level or concentration of DNA polymerase alpha and the rate of initiation of new rounds of DNA synthesis |
| 2632279 | Because cell size is inversely proportional to the concentration of DNA polymerase alpha and presumably other replication factors, it is inversely related to the initiation of new rounds of DNA synthesis |
| 3930524 | Induction of DNA polymerase alpha in senescent cultures of normal and Werner's syndrome cultured skin fibroblasts |
| 3930524 | DNA polymerase alpha activity was determined following serum stimulation of early and late passages of human diploid fibroblast-like (HDFL) cultures derived from apparently normal donors (two strains) and from a patient with Werner's syndrome (one strain) |
| 3930524 | The induction of DNA polymerase alpha in senescent cultures indicates that they retain the capacity to carry out some complex metabolic responses to mitogen stimulation |
| 3930524 | In addition, these results suggest the possibility that dilution of DNA polymerase alpha and/or other DNA replication factors may play a role in the onset or maintenance of the postmitotic state in the enlarged senescent HDFL cells |
| 7130287 | Evidence that a critical threshold of DNA polymerase-alpha activity may be required for the initiation of DNA synthesis in mammalian cell heterokaryons |
| 7130287 | Our results indicate that the specific activity of DNA polymerase-alpha (or some other factor tightly coregulated with it) in "recessive" cell types (those unable to rescue senescent cells) is only about two times this theoretical "threshold" value, and that fusion of recessive cell types to senescent HDF cells reduces the specific activity in the heterokaryon to below this minimum, thus preventing the cells from entering S phase |
| 7300456 | The first method produces DNA polymerase-alpha with a lower molecular weight and other properties that are not normally found for this enzyme |
| 7300456 | It was concluded that this method produces proteolytically degraded DNA polymerase-alpha |
| 7300456 | A second method was developed which produces DNA polymerase-alpha with all the normal properties of this enzyme |
| 7300456 | DNA polymerase-alpha accounts for 95% of polymerase activity in young cells and its specific activity during the fibroblast lifespan correlates with the declining cellular growth rate |
| 7300456 | The 5 S DNA polymerase-alpha has an increased in vitro error frequency (average 3 |
| 923389 | Apart from two exceptions found with lower organisms, the nuclear DNA polymerase activity was always higher under conditions which favoured the in vitro expression of DNA polymerase-beta rather than DNA polymerase-alpha |
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