| 26196672 | All cells were negative for leukocyte markers (CD19, CD34, CD45) and CD73 positive |
| 25437179 | When compared with N-UC-MSCs, GDM-UC-MSCs showed decreased cell growth and earlier cellular senescence with accumulation of p16 and p53, even though they expressed similar levels of CD105, CD90, and CD73 MSC marker proteins |
| 24170370 | 5 cc of bone marrow was needed to predictably isolate aBMSCs, and, regardless of methodology for harvest, cell-surface marker expression of CD73, CD90, CD105, and Stro-1 was similar for aBMSCs, being 89 |
| 23804221 | CONCLUSION: Our findings indicate that the combination of CD73 and CD39 allows the prospective purification from culture-expanded heterogeneous synovial MSC populations of a distinct MSC subset with greater chondro-osteogenic potency |
| 22242193 | Long term treatment did not induce lineage commitment in terms of osteogenic pathways but maintained their clonogenic capacity, their surface marker characteristics (expression of CD73, CD90, CD105) and their multipotency to develop towards the chondrogenic, adipogenic and osteogenic pathways |
| 21627568 | The surface expression of CD90 and CD73 was assessed by flow cytometry |
| 20225285 | The expressions of CD166, CD49a, and CD106 decreased, whereas those of CD10, CD29, CD44, CD73, CD90, and CD105 showed no significant change |
| 1805604 | 1b protein ratio, the stability of a number of glycolytic enzymes, and the instability of pyrimidine 5'-nucleotidase activity |
| 2154283 | Increased 5'-nucleotidase activity induced by dibutyryl cyclic AMP treatment of cultured glial cells |
| 2154283 | In an attempt to clarify the relationship between ecto-5'-nucleotidase (5'-N) activity and cell differentiation of glial cells, dibutyryl cyclic AMP (dBcAMP), which induces cell differentiation, was administrated to cultured rat glioma cells in logarithmic and confluent phases of cultivation |
| 2559327 | Enhancement of 5'-nucleotidase activity of human leukemic cells after fractionation: implications for cancer and aging |
| 2559327 | Previous studies reported that 5'-nucleotidase activity was undetectable or at much lower levels in the homogenate of human chronic lymphocytic leukemic (CCL) cells than in normal lymphocytes |
| 2559327 | In the present study, 5'-nucleotidase specific activity in acute myelocytic leukemia (AML), which varied in a range from undetectable to 1 |
| 2559327 | In a further fractionation of the cytosol of various leukemic cells with ammonium sulfate, 5'-nucleotidase specific activity increased up to 14-fold in the 60% (NH4)2SO4 fraction, with a recovery of 1266 +/- 115% |
| 2559327 | These data suggest that 5'-nucleotidase activity in fractionated leukemic cells is higher than reported previously and that the sum of 5'-nucleotidase activity in subcellular compartments is higher than that detected in the homogenate |
| 2559327 | Furthermore, even when 5'-nucleotidase was undetectable in a homogenate, it became detectable in the plasma membranes, suggesting that its ecto-enzyme function is still active in leukemic cells |
| 2559327 | The undetectable or low 5'-nucleotidase in the homogenate is indicative of (1) the enzyme itself being in an inactive form but becoming active after the fractionations, or (2) the presence of a factor(s) that prevents the enzyme from being detected but that is separated from the enzyme by the fractionations |
| 2559327 | In both cases, the rate of nucleotide catabolism by inactive 5'-nucleotidase in rapidly proliferating leukemic cells should be slower than when the enzyme is active |
| 2559327 | The present finding is consistent with our previous findings that during normal cell aging the high 5'-nucleotidase activity is associated with senescent non-proliferating cells but low or undetectable activity with rapidly proliferating immortal cells |
| 2559327 | The implications of 5'-nucleotidase for DNA synthesis in aging and cancer are discussed |
| 3021233 | Membrane-bound 5'-nucleotidase inhibitor in Ehrlich ascites tumour cells and newborn mouse liver |
| 3021233 | The cell homogenate, when mixed with adult mouse liver homogenate, inhibited the 5'-nucleotidase activity of the latter, without affecting its p-nitrophenyl phosphate-hydrolysing activity |
| 3021233 | From these results we conclude that a small molecular weight protein inhibitor(s) of 5'-nucleotidase is present in the plasma membrane of Ehrlich ascites tumour cells |
| 2998326 | Implications of 5'-nucleotidase and its inhibitor for cellular aging and cancer |
| 6307389 | Implications of a 5'-nucleotidase inhibitor in human leukemic cells for cellular aging and cancer |
| 6307389 | Preincubating the mixed samples for 1, 20 and 45 min, respectively, before adding the substrate, the heated soluble fraction of RPMI 8402 cells did not increase the percentage of inhibition for 5'-nucleotidase of the homogenate of IMR-90 cells |
| 6307389 | The present study suggest that the previously reported undetectability of 5'-nucleotidase in permanent cell lines could be due to the presence of a protein inhibitor for 5'-nucleotidase in these human leukemic cell lines |
| 6307389 | It also supports the hypothesis that the increased 5'-nucleotidase activity in normal senescent cells in vitro may be a control in cellular aging that is missing from leukemic cells in vitro |
| 449279 | In IMR-90 and CEF cells, both of which have a limited lifespan in vitro, 5'-nucleotidase activity increased 6- and 20-fold, respectively, with increasing population doublings |
| 449279 | Of the six cell lines tested, the conspicuous enhancement in 5'-nucleotidase activity with increasing population doublings was observed only in normal cell lines and was absent in transformed cell lines |
| 719132 | Absence of any detectable activity of the membrane marker enzyme 5'-nucleotidase in human red blood cells |
| 719132 | We have investigated in human red blood cell membranes the presence of a 5'-nucleotidase, which is considered a general marker of plasma membranes |
| 719132 | Thus, the ubiquitousness of 5'-nucleotidase in plasma membranes is questioned |
