HCSGD entry for MKRN1
1. General information
| Official gene symbol | MKRN1 |
|---|---|
| Entrez ID | 23608 |
| Gene full name | makorin ring finger protein 1 |
| Other gene symbols | RNF61 |
| Links to Entrez Gene | Links to Entrez Gene |
2. Neighbors in the network
This gene isn't in Literature mining network.
3. Gene ontology annotation
GO ID | GO term | Evidence | Category |
|---|---|---|---|
| GO:0003674 | Molecular_function | ND | molecular_function |
| GO:0003682 | Chromatin binding | IEA | molecular_function |
| GO:0005515 | Protein binding | IPI | molecular_function |
| GO:0005575 | Cellular_component | ND | cellular_component |
| GO:0008150 | Biological_process | ND | biological_process |
| GO:0008270 | Zinc ion binding | IEA | molecular_function |
| GO:0016567 | Protein ubiquitination | IEA | biological_process |
| GO:0016874 | Ligase activity | IEA | molecular_function |
| GO:0046872 | Metal ion binding | IEA | molecular_function |
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4. Expression levels in datasets
- Meta-analysis result
| p-value up | p-value down | FDR up | FDR down |
|---|---|---|---|
| 0.0934824057 | 0.9418608753 | 0.6407683333 | 1.0000000000 |
- Individual experiment result
( "-" represent NA in the specific microarray platform )
( "-" represent NA in the specific microarray platform )
| Data source | Up or down | Log fold change |
|---|---|---|
| GSE11954 | Up | 0.0758638787 |
| GSE13712_SHEAR | Down | -0.0716595721 |
| GSE13712_STATIC | Up | 0.0029294269 |
| GSE19018 | Up | 0.8021532336 |
| GSE19899_A1 | Up | 0.2071472704 |
| GSE19899_A2 | Up | 0.3555161945 |
| PubMed_21979375_A1 | Up | 0.1503453704 |
| PubMed_21979375_A2 | Up | 0.3939147545 |
| GSE35957 | Down | -0.1465550232 |
| GSE36640 | Up | 0.1119274964 |
| GSE54402 | Down | -0.0918275370 |
| GSE9593 | Up | 0.2315422902 |
| GSE43922 | Down | -0.0215340868 |
| GSE24585 | Up | 0.4994811527 |
| GSE37065 | Up | 0.2825955332 |
| GSE28863_A1 | Down | -0.0255928329 |
| GSE28863_A2 | Up | 0.1573942607 |
| GSE28863_A3 | Up | 0.0261322059 |
| GSE28863_A4 | Down | -0.1422892228 |
| GSE48662 | Up | 0.1725709819 |
5. Regulation relationships with compounds/drugs/microRNAs
- Compounds
Not regulated by compounds
- Drugs
Not regulated by drugs
- MicroRNAs
- mirTarBase
MiRNA_name | mirBase ID | miRTarBase ID | Experiment | Support type | References (Pubmed ID) |
|---|---|---|---|---|---|
| hsa-miR-373-3p | MIMAT0000726 | MIRT002511 | Microarray | Functional MTI (Weak) | 15685193 |
| hsa-miR-375 | MIMAT0000728 | MIRT019801 | Microarray | Functional MTI (Weak) | 20215506 |
| hsa-miR-574-3p | MIMAT0003239 | MIRT040519 | CLASH | Functional MTI (Weak) | 23622248 |
| hsa-miR-197-3p | MIMAT0000227 | MIRT048130 | CLASH | Functional MTI (Weak) | 23622248 |
| hsa-let-7c-5p | MIMAT0000064 | MIRT051838 | CLASH | Functional MTI (Weak) | 23622248 |
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- mirRecord
MicroRNA name | mirBase ID | Target site number | MiRNA mature ID | Test method inter | MiRNA regulation site | Reporter target site | Pubmed ID |
|---|---|---|---|---|---|---|---|
| hsa-miR-373-3p | MIMAT0000726 | NA | hsa-miR-373 | 15685193 |
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6. Text-mining results about the gene
Gene occurances in abstracts of cellular senescence-associated articles: 1 abstracts the gene occurs.
PubMed ID of the article | Sentenece the gene occurs |
|---|---|
| 23104211 | BACKGROUND: We investigated whether Makorin ring finger protein 1 (MKRN1), an E3 ligase, affects p14ARF-associated cellular senescence and tumorigenesis by posttranslational modification in gastric tumorigenesis |
| 23104211 | METHODS: A link between MKRN1 and ARF was examined in MKRN1 null mouse embryonic fibroblasts (MEFs) and in human fibroblasts and gastric cancer cells by silencing MKRN1 using small interfering RNA (siRNA) and short hairpin RNA (shRNA) |
| 23104211 | Ubiquitination and proteasomal degradation assays were used to assess p14ARF degradation associated with MKRN1 |
| 23104211 | MKRN1 and p14ARF expression levels were analyzed with immunohistochemistry in malignant and normal tissues from gastric cancer patients and with chi(2) tests |
| 23104211 | The tumor growth of gastric cancer cells stably expressing MKRN1 shRNA, p14ARF shRNA, or both was examined in mouse xenograft models (n = 4-6) and analyzed with unpaired t tests |
| 23104211 | RESULTS: MKRN1 knockout MEFs exhibited premature senescence and growth retardation with increased p19ARF protein expression |
| 23104211 | Similar results were obtained for human fibroblasts or gastric cancer cell lines by MKRN1 knockdown |
| 23104211 | Biochemical analyses confirmed that MKRN1 targets p14ARF for ubiquitination and subsequent proteasome-dependent degradation |
| 23104211 | A statistically significant association was shown between MKRN1 overexpression and p14ARF underexpression (P = |
| 23104211 | Xenograft analyses using p53-functional AGS or -dysfunctional SNU601 cells displayed statistically significant tumor growth retardation by silencing MKRN1, which was reversed under depletion of p14ARF (AGS cells, MKRN1 knockdown tumors vs MKRN1 and p14ARF knockdown tumors: 164 |
| 23104211 | CONCLUSIONS: We demonstrated that MKRN1 functions as a novel E3 ligase of p14ARF and that it potentially regulates cellular senescence and tumorigenesis in gastric cancer |
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