| 26749742 | Besides, the aggrecan and collagen type II expression were examined by toluidine blue and immunocytochemistry staining respectively |
| 26337541 | H2O2-senescent cells were found to possess a catabolic phenotype, mainly characterised by the up-regulation of extracellular matrix-degrading enzymes (MMP-1, -2, -9 and ADAMTS-5) and the down-regulation of their inhibitors (TIMPs), as well as of several proteoglycans, including aggrecan, the major component of the nucleus pulposus |
| 25826898 | Senescence marker (p16INK4a) and disc degeneration markers [A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS 5), Aggrecan, and Sry-related HMG box transcription factor 9 (Sox-9)] were determined in the NP specimens with immunohistochemistry and Western blot |
| 25826898 | The protein expressions of Aggrecan and Sox-9 in the NP with grade IV degeneration were significantly lower than those in the NP with grade III degeneration (P < 0 |
| 25087910 | Glucosamine attenuated the decrease of aggrecan and prevented the apoptosis of the NP cells induced by IL-1beta, whereas 3-MA partly reversed these effects |
| 23879090 | After corresponding treatment, Western blot was used for determining the protein expressions of SIRT1, Aggrecan, and collagen type II; real-time fluorescence quantitative PCR was used for detecting SIRT1 mRNA expression |
| 23879090 | The protein expressions of SIRT1, collagen type II, and Aggrecan in group B were significantly improved when compared with that in group A (P < 0 |
| 23438440 | Whole bovine discs were cultured to examine cellular (anabolic/catabolic gene expression, cell viability and senescence using beta-galactosidase) and structural (histology and aggrecan degradation) changes in response to TNFalpha treatment |
| 23438440 | Data indicates that TNFalpha increased aggrecan degradation products and suggests increased beta-galactosidase staining at 21 days without any recovery |
| 23262094 | Expression of the protease ADAMTS4 and aggrecan proteolytic fragments was significantly increased |
| 23238821 | The proteoglycan and collagen II in the extracellular matrix and the aggrecan and collagen II mRNA expression in NP cells of diabetic rats were decreased compared with the control group |
| 22531458 | Total disc proteoglycan (PG) content [1,9-dimethylmethylene blue (DMMB) assay], aggrecan proteolysis (immunobloting analysis), and cellular senescence (p16INK4a immunohistochemistry) were analyzed |
| 22531458 | Exposure to tobacco smoke dramatically increased metalloproteinase-mediated proteolysis of disc aggrecan within its interglobular domain (IGD) |
| 22531458 | Cleavage of aggrecan IGD is extremely detrimental as this results in the loss of the entire glycosaminoglycan-attachment region of aggrecan, which is vital for attracting water necessary to counteract compressive forces |
| 22454193 | The expression levels of mRNAs encoding aggrecan (ACAN), a major structural proteoglycan, type II collagen (COL2A1), and metalloproteinases (MMP) -1, -3, and -13, respectively, were determined using real-time PCR |
| 22454193 | RESULTS: Chondrocytes stimulated by IL-1beta showed increased MMP-1, MMP-3, and MMP-13 levels, whereas the expression of COL2A1 and ACAN decreased |
| 22454193 | However, in cells co-treated with IL-1beta and Rg3, the levels of MMP-1 and MMP-13 were lower than in cells treated with IL-1beta alone, and COL2A1 and ACAN expression levels recovered from the low values seen when cultured only in the presence of IL-1beta |
| 21627568 | Chondrogenesis, proteoglycan deposition, collagen type II protein expression, collagen type 2A1 (Col2AI), and aggrecan (Acan) mRNA expression were less in pellets formed with later passages of TDSCs after chondrogenic induction |
| 20533544 | OBJECTIVE: To determine whether intervertebral disc (IVD) cells express beta-catenin and to assess the role of the WNT/beta-catenin signaling pathway in cellular senescence and aggrecan synthesis |
| 20533544 | Activation of WNT/beta-catenin signaling also regulated the expression of aggrecan |
| 19058142 | After 7 days, DNA and sulfated glycosaminoglycan contents were analyzed along with real time, quantitative reverse transcription-polymerase chain reaction analysis for collagen types I and II, aggrecan, and matrix metalloproteinase-3 gene expression |
| 18024225 | Gene expression of types I, II, and VI collagen, aggrecan, and chondroitin-6-sulfotransferase in the human annulus: in situ hybridization findings |
| 18024225 | PURPOSE: To determine the percentage and patterns of gene expression for types I, II, and VI collagen, aggrecan, and chondroitin-6-sulfotransferase in the human annulus |
| 18024225 | OUTCOME MEASURES: The percentages of cells in the human annulus expressing type I, II, and VI collagen, aggrecan, and chondroitin-6-sulfotransferase |
| 18024225 | METHODS: In situ hybridization, a technique with high temporal and spatial resolution, was used to detect gene expression of types I, II, and VI collagen, aggrecan, and chondroitin-6 sulfotransferase in cells in adjacent sections of annulus from discs with Thompson grades of II, III, and IV |
| 18024225 | 8% of cells expressed aggrecan, 38 |
| 12721352 | As chondrocytes age, they synthesize smaller, less uniform aggrecan molecules and less functional link proteins, their mitotic and synthetic activity decline, and their responsiveness to anabolic mechanical stimuli and growth factors decreases |
