HCSGD entry for CDK6
1. General information
Official gene symbol | CDK6 |
---|---|
Entrez ID | 1021 |
Gene full name | cyclin-dependent kinase 6 |
Other gene symbols | PLSTIRE |
Links to Entrez Gene | Links to Entrez Gene |
2. Neighbors in the network
3. Gene ontology annotation
GO ID | GO term | Evidence | Category |
---|---|---|---|
GO:0000082 | G1/S transition of mitotic cell cycle | TAS | biological_process |
GO:0000278 | Mitotic cell cycle | TAS | biological_process |
GO:0000307 | Cyclin-dependent protein kinase holoenzyme complex | IDA | cellular_component |
GO:0001726 | Ruffle | IDA | cellular_component |
GO:0001954 | Positive regulation of cell-matrix adhesion | IDA | biological_process |
GO:0003323 | Type B pancreatic cell development | IDA | biological_process |
GO:0004693 | Cyclin-dependent protein serine/threonine kinase activity | IDA | molecular_function |
GO:0005515 | Protein binding | IPI | molecular_function |
GO:0005524 | ATP binding | IDA | molecular_function |
GO:0005634 | Nucleus | IDA | cellular_component |
GO:0005737 | Cytoplasm | IDA | cellular_component |
GO:0005829 | Cytosol | TAS | cellular_component |
GO:0006468 | Protein phosphorylation | IDA | biological_process |
GO:0007050 | Cell cycle arrest | TAS | biological_process |
GO:0008285 | Negative regulation of cell proliferation | TAS | biological_process |
GO:0009615 | Response to virus | IEP | biological_process |
GO:0010468 | Regulation of gene expression | IDA IMP | biological_process |
GO:0014002 | Astrocyte development | ISS | biological_process |
GO:0021542 | Dentate gyrus development | ISS | biological_process |
GO:0021670 | Lateral ventricle development | ISS | biological_process |
GO:0030332 | Cyclin binding | IPI | molecular_function |
GO:0042063 | Gliogenesis | IMP | biological_process |
GO:0043697 | Cell dedifferentiation | IMP | biological_process |
GO:0045596 | Negative regulation of cell differentiation | TAS | biological_process |
GO:0045638 | Negative regulation of myeloid cell differentiation | IDA | biological_process |
GO:0045646 | Regulation of erythrocyte differentiation | IMP | biological_process |
GO:0045668 | Negative regulation of osteoblast differentiation | IDA | biological_process |
GO:0045786 | Negative regulation of cell cycle | IDA | biological_process |
GO:0048146 | Positive regulation of fibroblast proliferation | IMP | biological_process |
GO:0048699 | Generation of neurons | ISS | biological_process |
GO:0050680 | Negative regulation of epithelial cell proliferation | IMP | biological_process |
GO:0051301 | Cell division | IEA | biological_process |
GO:2000145 | Regulation of cell motility | ISS | biological_process |
GO:2000773 | Negative regulation of cellular senescence | IDA | biological_process |
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4. Expression levels in datasets
- Meta-analysis result
p-value up | p-value down | FDR up | FDR down |
---|---|---|---|
0.3510113484 | 0.0209934805 | 0.9999902473 | 0.2804920812 |
- Individual experiment result
( "-" represent NA in the specific microarray platform )
( "-" represent NA in the specific microarray platform )
Data source | Up or down | Log fold change |
---|---|---|
GSE11954 | Down | -0.0559908463 |
GSE13712_SHEAR | Up | 0.3135134187 |
GSE13712_STATIC | Up | 0.0414567228 |
GSE19018 | Up | 0.6371590631 |
GSE19899_A1 | Down | -0.6713415552 |
GSE19899_A2 | Down | -0.5816241961 |
PubMed_21979375_A1 | Down | -0.5731280502 |
PubMed_21979375_A2 | Down | -0.6935374581 |
GSE35957 | Up | 0.9184850278 |
GSE36640 | Up | 0.4533960023 |
GSE54402 | Down | -0.7616159680 |
GSE9593 | Up | 0.1904910202 |
GSE43922 | Down | -0.4952636826 |
GSE24585 | Down | -0.7684793124 |
GSE37065 | Down | -0.2290882888 |
GSE28863_A1 | Down | -0.2208836768 |
GSE28863_A2 | Up | 0.8885805375 |
GSE28863_A3 | Down | -0.5529198244 |
GSE28863_A4 | Down | -0.2653049824 |
GSE48662 | Up | 0.1619736446 |
5. Regulation relationships with compounds/drugs/microRNAs
- Compounds
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- Drugs
Name | Drug | Accession number |
---|---|---|
Flavopiridol | DB03496 | EXPT00998 |
(2S)-2-({6-[(3-amino-5-chlorophenyl)amino]-9-isopropyl-9H-purin-2-yl}amino)-3-methylbutan-1-ol | DB07379 | - |
3,7,3',4'-TETRAHYDROXYFLAVONE | DB07795 | - |
Palbociclib | DB09073 | - |
- MicroRNAs
- mirTarBase
MiRNA_name | mirBase ID | miRTarBase ID | Experiment | Support type | References (Pubmed ID) |
---|---|---|---|---|---|
hsa-miR-195-5p | MIMAT0000461 | MIRT000224 | Reporter assay;Western blot;qRT-PCR;Other | Functional MTI | 19441017 |
hsa-let-7a-5p | MIMAT0000062 | MIRT000415 | CLASH | Functional MTI (Weak) | 23622248 |
hsa-miR-29a-3p | MIMAT0000086 | MIRT000682 | qRT-PCR//Western blot | Functional MTI | 19850741 |
hsa-miR-29a-3p | MIMAT0000086 | MIRT000682 | Luciferase reporter assay//qRT-PCR//Western blot//Reporter assay;Western blot;qRT-PCR | Functional MTI | 20086245 |
hsa-miR-29b-3p | MIMAT0000100 | MIRT000684 | qRT-PCR//Luciferase reporter assay//Western blot | Functional MTI | 19850741 |
hsa-miR-29b-3p | MIMAT0000100 | MIRT000684 | Luciferase reporter assay//qRT-PCR//Western blot//Reporter assay;Western blot;qRT-PCR | Functional MTI | 20086245 |
hsa-miR-34b-5p | MIMAT0000685 | MIRT000762 | Luciferase reporter assay | Functional MTI | 19461653 |
hsa-miR-424-5p | MIMAT0001341 | MIRT000938 | qRT-PCR//flow//Luciferase reporter assay//Western blot | Functional MTI | 18701644 |
hsa-miR-16-5p | MIMAT0000069 | MIRT000939 | qRT-PCR//flow//Luciferase reporter assay//Western blot | Functional MTI | 18701644 |
hsa-miR-26a-5p | MIMAT0000082 | MIRT006306 | Luciferase reporter assay//Western blot | Functional MTI | 22210897 |
hsa-miR-34a-5p | MIMAT0000255 | MIRT001012 | qRT-PCR//Luciferase reporter assay//Western blot//Reporter assay | Functional MTI | 18406353 |
hsa-miR-34a-5p | MIMAT0000255 | MIRT001012 | Luciferase reporter assay | Functional MTI | 19461653 |
hsa-miR-34a-5p | MIMAT0000255 | MIRT001012 | qRT-PCR//Western blot//Luciferase reporter assay | Functional MTI | 19584398 |
hsa-miR-34a-5p | MIMAT0000255 | MIRT001012 | Luciferase reporter assay//Western blot//Microarray | Functional MTI | 17914404 |
hsa-miR-34a-5p | MIMAT0000255 | MIRT001012 | Luciferase reporter assay//qRT-PCR//Western blot | Functional MTI | 19773441 |
hsa-miR-34a-5p | MIMAT0000255 | MIRT001012 | Western blot;Other | Functional MTI | 21240262 |
hsa-miR-107 | MIMAT0000104 | MIRT001233 | Western blot | Functional MTI | 19407485 |
hsa-miR-107 | MIMAT0000104 | MIRT001233 | Flow//Immunoblot//Microarray//qRT-PCR//Western blot | Functional MTI | 19688090 |
hsa-miR-107 | MIMAT0000104 | MIRT001233 | Luciferase reporter assay//Microarray//Western blot//qRT-PCR | Functional MTI | 20833636 |
hsa-miR-107 | MIMAT0000104 | MIRT001233 | Western blot;qRT-PCR | Functional MTI | 21264532 |
hsa-miR-30a-3p | MIMAT0000088 | MIRT001760 | Luciferase reporter assay//qRT-PCR//Western blot//Western blot;qRT-PCR | Functional MTI | 16239240 |
hsa-let-7b-5p | MIMAT0000063 | MIRT002297 | Luciferase reporter assay//Microarray//Western blot | Functional MTI | 17699775 |
hsa-miR-21-5p | MIMAT0000076 | MIRT002406 | Sequencing | Functional MTI (Weak) | 20371350 |
hsa-miR-137 | MIMAT0000429 | MIRT002490 | Luciferase reporter assay//qRT-PCR//Western blot | Functional MTI | 18381414 |
hsa-miR-137 | MIMAT0000429 | MIRT002490 | Luciferase reporter assay | Functional MTI | 18577219 |
hsa-miR-137 | MIMAT0000429 | MIRT002490 | Western blot | Functional MTI | 20682795 |
hsa-miR-137 | MIMAT0000429 | MIRT002490 | Luciferase reporter assay//qRT-PCR//Western blot | Functional MTI | 21051724 |
hsa-miR-137 | MIMAT0000429 | MIRT002490 | Luciferase reporter assay | Functional MTI | 23178712 |
hsa-miR-124-3p | MIMAT0000422 | MIRT002642 | ChIP-seq//Western blot | Functional MTI | 19435910 |
hsa-miR-124-3p | MIMAT0000422 | MIRT002642 | Immunohistochemistry//Luciferase reporter assay//Microarray//qRT-PCR//Western blot | Functional MTI | 17308079 |
hsa-miR-124-3p | MIMAT0000422 | MIRT002642 | Luciferase reporter assay//Western blot | Functional MTI | 18607543 |
hsa-miR-124-3p | MIMAT0000422 | MIRT002642 | Luciferase reporter assay | Functional MTI | 17581274 |
hsa-miR-124-3p | MIMAT0000422 | MIRT002642 | Luciferase reporter assay | Functional MTI | 18577219 |
hsa-miR-124-3p | MIMAT0000422 | MIRT002642 | Luciferase reporter assay//qRT-PCR//Western blot | Functional MTI | 19843643 |
hsa-miR-124-3p | MIMAT0000422 | MIRT002642 | Microarray | Functional MTI (Weak) | 18668037 |
hsa-miR-124-3p | MIMAT0000422 | MIRT002642 | Microarray | Functional MTI (Weak) | 15685193 |
hsa-miR-29c-3p | MIMAT0000681 | MIRT003200 | Luciferase reporter assay//qRT-PCR//Western blot//Reporter assay;Western blot;qRT-PCR;Other | Functional MTI | 20086245 |
hsa-miR-449a | MIMAT0001541 | MIRT003187 | Luciferase reporter assay//Microarray//qRT-PCR//Western blot//Reporter assay;Western blot;Other | Functional MTI | 19833767 |
hsa-miR-26b-5p | MIMAT0000083 | MIRT006307 | Luciferase reporter assay//Western blot | Functional MTI | 22210897 |
hsa-miR-34b-3p | MIMAT0004676 | MIRT003579 | Luciferase reporter assay//Western blot | Functional MTI | 18768788 |
hsa-miR-185-5p | MIMAT0000455 | MIRT004700 | Flow//Immunoblot//Microarray//qRT-PCR//Western blog | Functional MTI | 19688090 |
hsa-miR-185-5p | MIMAT0000455 | MIRT004700 | CLASH | Functional MTI (Weak) | 23622248 |
hsa-miR-203a | MIMAT0000264 | MIRT004723 | Luciferase reporter assay//qRT-PCR//Western blot | Functional MTI | 19843643 |
hsa-miR-191-5p | MIMAT0000440 | MIRT006721 | Luciferase reporter assay//qRT-PCR//Western blot | Functional MTI | 22683624 |
hsa-miR-218-5p | MIMAT0000275 | MIRT007145 | HITS-CLIP//Luciferase reporter assay//Western blot | Functional MTI | 23212916 |
hsa-miR-449b-5p | MIMAT0003327 | MIRT016139 | Reporter assay;Western blot | Functional MTI | 19833767 |
hsa-miR-193b-3p | MIMAT0002819 | MIRT016513 | Microarray | Functional MTI (Weak) | 20304954 |
hsa-miR-103a-3p | MIMAT0000101 | MIRT027234 | Sequencing | Functional MTI (Weak) | 20371350 |
hsa-miR-22-3p | MIMAT0000077 | MIRT030654 | Sequencing | Functional MTI (Weak) | 20371350 |
hsa-miR-1303 | MIMAT0005891 | MIRT035884 | CLASH | Functional MTI (Weak) | 23622248 |
hsa-miR-615-3p | MIMAT0003283 | MIRT040000 | CLASH | Functional MTI (Weak) | 23622248 |
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- mirRecord
MicroRNA name | mirBase ID | Target site number | MiRNA mature ID | Test method inter | MiRNA regulation site | Reporter target site | Pubmed ID |
---|---|---|---|---|---|---|---|
hsa-miR-137 | MIMAT0000429 | 1 | hsa-miR-137 | {Western blot} | {overexpression by miRNA precursor transfection} | 18381414 | |
hsa-miR-21-5p | MIMAT0000076 | NA | hsa-miR-21 | {Western blot} | {overexpression by miRNA precursor transfection} | 17991735 | |
hsa-let-7b-5p | MIMAT0000063 | NA | hsa-let-7b | {Western blot} | {overexpression by miRNA precursor transfection} | 17699775 | |
hsa-miR-16-5p | MIMAT0000069 | NA | hsa-miR-16 | {Western blot} | {overexpression} | 18701644 | |
hsa-miR-34a-5p | MIMAT0000255 | NA | hsa-miR-34a | {Western blot} | {overexpression by miRNA precursor transfection} | 18834855 | |
hsa-miR-125b-5p | MIMAT0000423 | NA | hsa-miR-125b | {Western blot} | {overexpression} | 19948152 | |
hsa-miR-107 | MIMAT0000104 | NA | hsa-miR-107 | {Western blot} | {overexpression by miRNA mimics tranfection} | 19688090 | |
hsa-miR-185-5p | MIMAT0000455 | NA | hsa-miR-185 | 19688090 | |||
hsa-miR-29a-3p | MIMAT0000086 | NA | hsa-miR-29a | {Western blot} | {overexpression} | 19850741 | |
hsa-miR-29b-3p | MIMAT0000100 | 2 | hsa-miR-29b | {Western blot} | {overexpression} | 19850741 | |
hsa-miR-29b-3p | MIMAT0000100 | 3 | hsa-miR-29b | {Western blot} | {overexpression} | 19850741 | |
hsa-miR-29b-3p | MIMAT0000100 | 1 | hsa-miR-29b | {Western blot} | {overexpression} | 19850741 | |
hsa-miR-195-5p | MIMAT0000461 | 1 | hsa-miR-195 | {Western blot} | {overexpression} | 19441017 | |
hsa-miR-195-5p | MIMAT0000461 | 2 | hsa-miR-195 | {Western blot} | {overexpression} | 19441017 | |
hsa-miR-129-5p | MIMAT0000242 | NA | hsa-miR-129-5p | {Western blot} | {overexpression} | 20404570 |
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6. Text-mining results about the gene
Gene occurances in abstracts of cellular senescence-associated articles: 16 abstracts the gene occurs.
PubMed ID of the article | Sentenece the gene occurs |
---|---|
27206849 | Here, we report that activation of the cyclin-dependent kinases CDK4 and CDK6 are essential and sufficient to impair senescence induced by PML expression |
27059142 | MicroRNA-33 promotes the replicative senescence of mouse embryonic fibroblasts by suppressing CDK6 |
27059142 | Moreover, we verified CDK6 as a direct target of microRNA-33 in mouse |
27059142 | Silencing of CDK6 induced the premature senescence phenotype of MEFs similarly as microRNA-33, while enforced expression of CDK6 significantly reverse the senescence-induction effect of microRNA-33 |
27059142 | Taken together, our results suggested that microRNA-33 enhanced the replicative senescence of MEFs potentially by suppressing CDK6 expression |
25684390 | Furthermore, in silico analysis identified four possible miR-433 target genes associated with cellular senescence: cyclin-dependent kinase 6 (CDK6), MAPK14, E2F3, and CDKN2A |
25684390 | Mechanistically, we demonstrate that downregulation of p-Rb is attributable to a miR-433-dependent downregulation of CDK6, establishing it as a novel miR-433 associated gene |
25255445 | Specifically, following siRNA knockdown, quantitative PCR, western blot analyses and chromatin immunoprecipitation (ChIP) show that NF-kappaB2 regulates the expression of CDK4 and CDK6, while RelB, through the regulation of genes such as PSMA5 and ANAPC1, regulates the stability of p21WAF1 and the tumour suppressor p53 |
24046371 | This oncogenic p16(INK4A) activity depends on inhibition of CDK4/CDK6, suggesting that in cervical cancer cells where retinoblastoma tumor suppressor is inactivated, CDK4/CDK6 activity needs to be inhibited in order for cells to survive |
23576552 | Furthermore, we show that Cdk6 is a direct downstream target gene of YAP in the regulation of cellular senescence, and the expression of Cdk6 is through the YAP-TEAD complex |
23576552 | Ectopic expression of Cdk6 rescued YAP knockdown-induced senescence |
23576552 | Finally, we find that downregulation of YAP in tumor cells increases senescence in response to chemotherapeutic agents, and YAP or Cdk6 expression rescues cellular senescence |
22820504 | The expression of p16(INK4A) was significantly increased, whereas levels of CDK4, CDK6 and p-Rb expression were decreased in the MSCs from both untreated and treated SLE patients |
21047732 | Paradoxically, G(1)-S phase genes such as MYC, CDK4, CDK6, CCND1, and CCND2 were strongly downregulated, in line with the observed G(1) arrest |
19098430 | In contrast, the levels of cyclin D1, CDK4 and CDK6 were sharply decreased |
18843795 | We also show that overexpression of CDK4, or its homologue CDK6, but not the downstream kinase, CDK2, inhibited the ability of wild-type p16(INK4a) to promote cell cycle arrest and senescence |
15460900 | The p16INK4a tumor suppressor protein functions as an inhibitor of CDK4 and CDK6, the D-type cyclin-dependent kinases that initiate the phosphorylation of the retinoblastoma tumor suppressor protein, RB |
9732051 | Likewise, cyclin D is complexed with its catalytic partners CDK4 and CDK6 in senescent HDF, but it is not known whether these complexes are active |
9732051 | In contrast, p16Ink4a, which binds monomeric CDK4 and CDK6 thereby preventing their binding to cyclin D, is increased dramatically at the time of senescence and remains high for at least 2 mo |
9704925 | There was also an increased binding of p16 to the Cdk6 kinase in senescent cells, and a decreased Cdk6 as well as Cdk2 kinase activity |
9584157 | Cyclin D2 was also found to be associated with the cyclin-dependent kinases CDK2 and CDK4 but not CDK6 during growth arrest |
9395243 | The p16/MTS1/CDKN2 gene on human chromosome band 9p21 encodes two unrelated proteins: p16INK4a, a specific inhibitor of the cyclin D-dependent kinases CKD4 and CDK6, and the structurally unrelated p19ARF protein that arrests cell growth in G1/S and also in G2/M |
8943005 | To determine a possible mechanism by which senescent human fibroblasts maintain a hypophosphorylated Rb, we examined the expression levels and interaction of the Rb kinases, CDK4 and CDK6, and the cyclin-dependent kinase inhibitors p21 and p16 in senescent HDFs |
8943005 | In senescent HDFs, p16 was shown to be complexed to both CDK4 and CDK6 |
8943005 | Immunodepletion analysis of p21 and p16 from the senescent cell extracts revealed that p16 is the major CDK inhibitor for both CDK4 and CDK6 kinases |
8943005 | Immunoprecipitation of CDK4 and CDK6 and their associated proteins from radiolabeled extracts from senescent HDFs showed no other CDK inhibitors |
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